An enzyme-linked immunosorbant assay using polyclonal antibodies against bacopaside I

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• 作者：Watoo Phrompittayarat ; Waraporn Putalun ; Hiroyuki Tanaka ; Sakchai Wittaya-Areekul ; Kanchalee Jetiyanon ; Kornkanok Ingkaninan
• 关键词：Bacopaside I ; Bacopa monnieri ; Enzyme-linked immunosorbant assay (ELISA) ; Pseudojujubogenin glycosides ; Polyclonal antibodies
• 刊名：Analytica Chimica Acta
• 出版年：2007
• 出版时间：12 February 2007
• 年：2007
• 卷：584
• 期：1
• 页码：1-6
• 全文大小：470 K

文摘

Bacopa monnieri (L.) Wettst. (Brahmi) is a medicinal plant used as a memory enhancer in Ayurvedic medicines. Its active components are triterpenoid glycosides namely pseudojujubogenin and jujubogenin glycosides. In order to analyze these saponin glycosides, an enzyme-linked immunosorbant assay (ELISA) was developed using polyclonal antibodies against bacopaside I, one of the pseudojujubogenin glycosides found in the plant. Bacopaside I was conjugated with a bovine albumin serum (BSA) to prepare an immunogen. The bacopaside I–BSA conjugate was immunized to a rabbit for producing polyclonal antibodies (PAbs). The results showed that the antibodies were raised specifically against pseudojujubogenin glycosides. An ELISA using anti-bacopaside I PAbs was performed in the range of 1.95–62.5 ng mL⁻¹ of bacopaside I and the limit of detection was 0.1 ng mL⁻¹. The method was validated and the applicability of the ELISA for analyzing saponin glycosides from Brahmi was demonstrated.