Crystal Structure of Cpf1 in Complex with Guide RNA and Target DNA
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- 作者：Takashi Yamano1 ; 11 ; Hiroshi Nishimasu1 ; 2 ; 11 ; Bernd Zetsche3 ; 4 ; 5 ; 6 ; 7 ; Hisato Hirano1 ; Ian M. Slaymaker3 ; 4 ; 5 ; 6 ; Yinqing Li3 ; 4 ; 5 ; 6 ; Iana Fedorova3 ; 4 ; 5 ; 6 ; 8 ; 9 ; Takanori Nakane1 ; Kira S. Makarova10 ; Eugene V. Koonin10 ; Ryuichiro Ishitani1 ; Feng Zhang3 ; 4 ; 5 ; 6 ; zhang@broadinstitute.org" class="auth_mail" title="E-mail the corresponding author ; Osamu Nureki1 ; nureki@bs.s.u-tokyo.ac.jp" class="auth_mail" title="E-mail the corresponding author
- 刊名：Cell
- 出版年：2016
- 出版时间：5 May 2016
- 年：2016
- 卷：165
- 期：4
- 页码：949-962
- 全文大小：6347 K
文摘
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Crystal structure of Acidaminococcus sp. Cpf1 in complex with crRNA and target DNA
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Mechanistic insights into Cpf1-induced, staggered DNA double-strand breaks
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Recognition of the 5′-TTTN-3′ PAM via base and shape readout mechanisms
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Striking similarity and major differences between the structures of Cpf1 and Cas9