Generation of antigen-specific cytotoxic T lymphocytes with activated B cells
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- 作者：Sun Ok Yun<sup>1sup> ; Hee Young Shin<sup>1sup> ; Chang-Yuil Kang<sup>2sup> ; Hyoung Jin Kang<sup>1sup><sup> ; sup><sup>kanghj@snu.ac.krsup>
- 关键词：B cells ; DNA nucleofection ; α-galactosylceramide (α-GalCer) ; cytotoxic T lymphocytes (CTLs)
- 刊名：Cytotherapy
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：19
- 期：1
- 页码：119-127
- 全文大小：1189 K
- 卷排序：19
文摘
Dendritic cells are well known as the most potent antigen-presenting cells. Nonetheless, their use in immunotherapy has been limited by the time-consuming and laborious steps involved in their generation in vitro. Therefore, much attention has been placed on alternative antigen-presenting cells that are relatively more convenient to manipulate.MethodsIn this study, the efficacy of B cells as antigen-presenting cells, compared with dendritic cells, in the induction of cytotoxic T lymphocytes against cytomegalovirus-specific antigens was evaluated. B cells were isolated from the peripheral blood mononuclear cells of healthy individuals, loaded with α-galactosylceramide for activation, and nucleofected with cytomegalovirus-antigen coding plasmid DNA. Antigen-nucleofected B cells or dendritic cells were cocultured with T cells for 14 days in vitro.ResultsThe proliferation of cytotoxic T lymphocytes induced by B cells was similar to that of those induced by dendritic cells. Additionally, the immunogenicity of both sets of cytotoxic T lymphocytes was similar not only in interferon-γ enzyme-linked immunospot assays but also in cytotoxicity assays.DiscussionThese observations suggest that α-galactosylceramide-loaded B cells could be used as antigen-presenting cells as an alternative to dendritic cells. Using B cells has several benefits, including cost-effectiveness and being both less time-consuming and less labor-intensive.