Erratum to “Identification of a novel endochitinase from a marine bacterium Vibrio proteolyticus strain No. 442” [Biochim. Biophys. Acta 1774 (2007) 1099–1107]
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- 作者：Shiro Itoi ; Yuna Kanomata ; Yuki Koyama ; Kazunari Kadokura ; Shinsuke Uchida ; Toshiyuki Nishio ; Tadatake Oku ; Haruo Sugita
- 关键词：Mouse cumulus– ; oocyte complex ; Two-dimensional electrophoresis ; Mass spectrometry ; Protein profile
- 刊名：Biochimica et Biophysica Acta - Proteins and Proteomics
- 出版年：2007
- 出版时间：November 2007
- 年：2007
- 卷：1774
- 期：11
- 页码：1491
- 全文大小：71 K
文摘
In mammals, the cumulus–oocyte complex (COC) is the main component of ovarian follicles. Bi-directional communication between oocytes and surrounding cumulus granulosa cells is essential for the development of oocytes and ovarian follicles. In this study, we performed proteomic profiling of mouse mature COC, using two-dimensional gel electrophoresis and mass spectrometry. A total of 259 protein spots were identified, which correspond to 156 individual proteins. We also discovered some protein families, which may play important roles in ovarian follicular development. Immunostaining was conducted to determine the subcellular localization of specific proteins from selected protein families of interest, and to examine their expression patterns during follicle development. These data provide valuable information for future studies to identify proteins involved in ovarian follicular development and related reproductive abnormalities.