Trichobilharzia regenti (Schistosomatidae): 3D imaging techniques in characterization of larval migration through the CNS of vertebrates
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- 作者：Jana Bulantová ; a ; bulantov@natur.cuni.cz" class="auth_mail" title="E-mail the corresponding author ; Tomá ; &scaron ; Machá ; čeka ; tomas.machacek@natur.cuni.cz" class="auth_mail" title="E-mail the corresponding author ; Lucie Panská ; a ; lichten@centrum.cz" class="auth_mail" title="E-mail the corresponding author ; Franti&scaron ; ek Krejčí ; b ; frantisek.krejci@utef.cvut.cz" class="auth_mail" title="E-mail the corresponding author ; Jakub Karchb ; jakub.karch@utef.cvut.cz" class="auth_mail" title="E-mail the corresponding author ; Nina Jä ; hrlingc ; d ; nina.jaehrling@tuwien.ac.at" class="auth_mail" title="E-mail the corresponding author ; Saiedeh Saghafic ; d ; saiedeh.saghafi@tuwien.ac.at" class="auth_mail" title="E-mail the corresponding author ; Hans-Ulrich Dodtc ; d ; hans-ulrich.dodt@meduniwien.ac.at" class="auth_mail" title="E-mail the corresponding author ; Petr Horá ; ka ; petrhorak@petrhorak.eu" class="auth_mail" title="E-mail the corresponding author
- 关键词：2D ; two-dimensional space ; 3D ; three-dimensional space ; CFSE ; carboxyfluorescein succinimidyl ester ; CNS ; central nervous system ; CPD ; critical point dryer ; CT ; computed tomography ; d. p. i. ; days post infection ; EDTA ; ethylene diamine tetraacetic acid ; EPMA ; 2.5% solution of phosphomolybdic acid in 70% EtOH ; EPTA ; 2.5% solution of phosphotungstic acid in 70% EtOH ; FMT ; fluorescence molecular tomography ; GA ; glutaraldehyde ; GFP ; green fluorescent protein ; LSFM ; light sheet fluorescent microscopy
- 刊名：Micron
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：83
- 期：Complete
- 页码：62-71
- 全文大小：2462 K
文摘
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Larvae of bird schistosomes can be tracked by use of intravital fluorescent dyes.
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3D imaging of schistosomes in tissues can be done by ultramicroscopy or micro-CT.
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These methods contribute to our knowledge of schistosome pathogenicity.