Stem cell repair of physeal cartilage

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• 作者：Ahn ; Jae I. ; Terry Canale ; S. ; Butler ; Stephanie D. ; Hasty ; Karen A.
• 关键词：Mesenchymal stem cells ; Bone bridge ; Growth arrest ; Physis
• 刊名：Journal of Orthopaedic Research
• 出版年：2004
• 出版时间：November, 2004
• 年：2004
• 卷：22
• 期：6
• 页码：1215-1221
• 全文大小：607 K

文摘

To evaluate the ability of cultured mesenchymal stem cells (MSC) to repair physeal defects, MSC-matrix constructs with 5 % gelatin (group A), 10 % gelatin/Gelfoam (Pharmacia, Peapack, NJ) (group B), and MSC grown in the presence of TGF-β₃ with Gelfoam (group C) were implanted in proximal tibial physeal defects created in 20 immature rabbits. Control groups (untreated partial defect and partial defect treated with Gelfoam) showed bony bar formation with varus deformities of 30° and 28°, respectively. Group A had an average 23° varus deformity with bony bridge formation, and group B had mild varus angulation (average 14°) of the proximal tibia. In group C, there was no significant varus deformity (average 9°), and histologic examination showed that some of the columnation areas interspersed with chondrocytes were irregularly arranged in the matrix. These findings suggest that repair of physeal defects can be enhanced by the implantation of MSC cultured with TGF-β₃.