SV40-mediated immortalization of human fibroblasts
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- 作者：Ozer ; Harvey L. ; Banga ; Satnam S. ; Dasgupta ; Tanya ; Houghton ; JeanMarie ; Hubbard ; Karen ; Jha ; Krishna K. ; et. al.
- 关键词：SV40 ; immortalization ; transformed human fibroblasts ; chromosome 6 ; SEN6 ; senescence ; growth suppressor ; T antigen ; RB ; p53
- 刊名：Experimental Gerontology
- 出版年：1996
- 出版时间：January 4, 1996
- 年：1996
- 卷：31
- 期：1-2
- 页码：303-310
- 全文大小：643 K
文摘
We have identified a multistep mechanism by which the DNA virus SV40 overcomes cellular senescence. Expression of SV40 T antigen is required for both transient extension of life span and unlimited life span or immortalization. These effects are mediated through inactivation of function of growth suppressors pRB and p53 via complex formation with T antigen. However, immortalization additionally requires inactivation of a novel growth suppressor gene, which has recently been identified to be on the distal portion of the long arm of chromosome 6, designatedSEN6 . We propose that SEN6 is responsible for cellular senescence in fibroblasts and other cells.