Characterization of extracts from winery by-products with antifungal activity against Botrytis cinerea

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• 作者：Leonora Mendoza ; Karen Ya?ez ; Marcela Vivanco ; Ricardo Melo ; Milena Cotoras
• 关键词：Grape pomace ; Antifungal activity ; Botrytis cinerea ; Phenolic compounds
• 刊名：Industrial Crops and Products
• 出版年：2013
• 出版时间：May, 2013
• 年：2013
• 卷：43
• 期：Complete
• 页码：360-364
• 全文大小：241 K

文摘

Phenolic compound profiles of grape pomace extracts from a mixture of Chilean grape varieties (Cabernet Sauvignon, Carm¨¨nere and Syrah) were analyzed. Two extraction methods were used: solvent and soxhlet extraction, followed by fractionation with hexane, chloroform and ethyl acetate. Phenolic compounds in different fractions were identified by HPLC. Ethyl acetate phase of solvent extraction contained a wide variety of phenolic compounds. Hexane phase of the extracts presented lowest diversity. Quercetin was found in almost all fractions.

Also, the in vitro antifungal activity of these extracts against the phytopathogenic fungus Botrytis cinerea was evaluated. Hexane or chloroform fractions from extracts obtained by solvent extraction showed the highest inhibitory effect on mycelia growth of this fungus, with IC₅₀ value of 40 ppm. In general, ethyl acetate fractions were less active against B. cinerea. Therefore, it can be concluded that grape pomace are a good low cost source to obtain antifungal extracts.