Structural determination and NMR characterization of a bacterial exopolysaccharide

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• 作者：Imen Dahech^a ; ^{imenbiologie@yahoo.fr} ; Jawhar Fakhfakh^b ; Mohamed Damak^b ; Hafedh Belghith^c ; Hafedh Mejdoub^a ; Karima Srih Belghith^a
• 关键词：Bacillus licheniformis ; Exopolysaccharide ; Structural analysis ; NMR spectroscopy
• 刊名：International Journal of Biological Macromolecules
• 出版年：2013
• 出版时间：August, 2013
• 年：2013
• 卷：59
• 期：Complete
• 页码：417-422
• 全文大小：1551 K

文摘

A strain of Bacillus licheniformis with high exopolysaccharide (EPS) production ability was isolated and identified. A new type of EPS was isolated from the strain fermentation and its structural characteristics were investigated and elucidated by partial and total acid hydrolysis, Fourier transform infrared, and ¹H and ¹³C NMR spectroscopy including 2D ¹H, COSY, NOESY, XHCOR and HMBC experiments. Based on obtained data, the EPS was found to be a levan composed of linear chains of (2 ¡ú 6)-linked ¦Â-d-fructofuranosyl residues with connections ¦Â (2 ¡ú 6).