Modification and cross-linking parameters in hyaluronic acid hydrogels¡ªDefinitions and analytical methods

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• 作者：Lennart Kenne ; Suresh Gohil ; Eva M. Nilsson ; Anders Karlsson ; David Ericsson ; Anne Hel ; er Kenne ; Lars I. Nord
• 关键词：MoD ; degree of modification ; DS ; degree of substitution ; CrD ; degree of cross-linking by stoichiometric relationship ; DC ; degree of cross-linking by fraction cross-linked monomer units ; CrR ; effective cross-linker ratio
• 刊名：Carbohydrate Polymers
• 出版年：2013
• 出版时间：2 January, 2013
• 年：2013
• 卷：91
• 期：1
• 页码：410-418
• 全文大小：1111 K

文摘

Definitions and methods for the quantification of degree of modification and cross-linking in cross-linked hyaluronic acid (HA) hydrogels are outlined. A novel method is presented in which the HA hydrogel is degraded by the enzyme chondroitinase AC and the digest product analyzed by size exclusion chromatography combined with electrospray ionization mass spectrometry (SEC-ESI-MS). This method allows for the determination of effective cross-linker ratio (CrR) which together with the degree of modification (MoD), determined by, e.g. ¹H NMR spectroscopy, enables the calculation of the degree of substitution (DS) and degree of cross-linking (CrD).

The method, could be applicable to the major cross-linked HA hydrogels currently on the market, and is exemplified here by application to two HA hydrogels. The definitions and methods presented are important contributions in attempts to find relationships between MoD, DS and CrD to mechanical properties as well as to biocompatibility of HA hydrogels.