Nuclear Receptor-Enhanced Transcription Requires Motor- and LSD1-Dependent Gene Networking in Interchromatin Granules
详细信息 查看全文
- 作者：Esperanza Nunez ; Young-Soo Kwon ; Kasey R. Hutt ; Qidong Hu ; Maria Dafne Cardamone ; Kenneth A. Ohgi ; Ivan Garcia-Bassets ; David W. Rose ; Christopher K. Glass ; Michael G. Rosenfeld ; Xiang-Dong Fu
- 关键词：CELLBIO ; DNA ; SIGNALING
- 刊名：Cell
- 出版年：2008
- 出版时间：21 March 2008
- 年：2008
- 卷：132
- 期：6
- 页码：996-1010
- 全文大小：2232 K
文摘
While the transcriptional machinery has been extensively dissected at the molecular level, little is known about regulation of chromosomal organization in the three-dimensional space of the nucleus to achieve integrated transcriptional responses to diverse signaling events. Here, we report that ligand induces rapid interchromosomal interactions among subsets of estrogen receptor ![]()
rc=""http://www.sciencedirect.com/scidirimg/entities/204e.gif"" alt=""greek small letter alpha"" title=""greek small letter alpha"" border=""0"">-bound transcription units, with a dramatic reorganization of nuclear territories requiring nuclear actin/myosin-I transport machinery, dynein light chain 1 (DLC1), and a specific subset of transcriptional coactivators and chromatin remodeling complexes. We establish a requirement for the histone lysine demethylase, LSD1, in directing specific interchromosomal interaction loci to distinct interchromatin granules, long thought to be “storage” sites for splicing machinery, and demonstrate that these three-dimensional motor-dependent interactions are required to achieve enhanced transcription of specific estrogen-receptor target genes. These findings reveal roles for the modulation of nuclear architecture in orchestrating regulated gene-expression programs in the mammalian nucleus.