A manganese superoxide dismutase (MnSOD) from Ruditapes philippinarum: Comparative structural- and expressional-analysis with copper/zinc superoxide dismutase (Cu/ZnSOD) and biochemical analysis of its antioxidant activities

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• 作者：Navaneethaiyer Umasuthan ; S.D.N.K. Bathige ; Kasthuri Saranya Revathy ; Youngdeuk Lee ; Ilson Whang ; Cheol Young Choi ; Hae-Chul Park ; Jehee Lee
• 关键词：Ruditapes philippinarum ; Manganese superoxide dismutase (MnSOD) ; Copper/znic superoxide dimutase (Cu/ZnSOD) ; Gene expression ; Antioxidant activity
• 刊名：Fish & Shellfish Immunology
• 出版年：2012
• 出版时间：October, 2012
• 年：2012
• 卷：33
• 期：4
• 页码：753-765
• 全文大小：2312 K

文摘

Superoxide dismutases (SODs), antioxidant metalloenzymes, represent the first line of defense in biological systems against oxidative stress caused by excessive reactive oxygen species (ROS), in particular . Two distinct members of SOD family were identified from Manila clam Ruditapes philippinarum (abbreviated as RpMnSOD and RpCu/ZnSOD). The structural analysis revealed all common characteristics of SOD family in both RpSODs from primary to tertiary levels, including three MnSOD signatures and two Cu/ZnSOD signatures as well as invariant Mn²⁺- and Cu/Zn²⁺-binding sites in RpMnSOD and RpCu/ZnSOD, respectively. Putative RpMnSOD and RpCu/ZnSOD proteins were predicted to be localized in mitochondrial matrix and cytosol, respectively. They shared 65.2 % and 63.9 % of identity with human MnSOD and Cu/ZnSOD, respectively. Phylogentic evidences indicated the emergence of RpSODs within molluscan monophyletic clade. The analogous spatial expression profiles of RpSODs demonstrated their higher mRNA levels in hemocytes and gills. The experimental challenges with poly I:C, lipopolysaccharide and Vibrio tapetis illustrated the time-dependent dynamic expression of RpSODs in hemocytes and gills. The recombinant RpMnSOD was expressed in a prokaryotic system and its antioxidant property was studied. The rRpMnSOD exhibited its optimum activity at 20?¡ãC, under alkaline condition (pH 9) with a specific activity of 3299?U?mg^?1. These outcomes suggested that RpSODs were constitutively expressing inducible proteins that might play crucial role(s) in innate immunity of Manila clam.