Stress-induced phosphorylation of SNAP-25

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• 作者：Saori Yamamori ; Daichi Sugaya ; Yuuki Iida ; Hirotoshi Kokubo ; Makoto Itakura ; Eiji Suzuki ; Masakazu Kataoka ; Hitoshi Miyaoka ; Masami Takahashi
• 关键词：HPA-axis ; hypothalamic-pituitary-adrenal-axis ; SNAP-25 ; synaptosomal-associated protein of 25  ; kDa
• 刊名：Neuroscience Letters
• 出版年：21 February, 2014
• 年：2014
• 卷：561
• 期：Complete
• 页码：182-187
• 全文大小：1373 K

文摘

Synaptosomal-associated protein of 25 kDa (SNAP-25), a t-SNARE protein, plays a crucial role in neurotransmitter release by exocytosis. Protein kinase C phosphorylates SNAP-25 at Ser¹⁸⁷, however the physiological significance of this phosphorylation event in brain function remains unclear. In the present study, we found that SNAP-25 phosphorylation increased rapidly in the mouse brain following cold-water restraint stress. Both basal and stress-induced phosphorylation of SNAP-25 were high in stress-related brain regions, including the cerebral cortex, hippocampus, and amygdala, and the extent of phosphorylation increased with increasing amounts of stress. Intravenous administration of adrenaline increased SNAP-25 phosphorylation, although stress-induced phosphorylation was still observed in adrenalectomized mice. These results indicate that SNAP-25 phosphorylation is regulated in a stress-dependent manner through both central and peripheral mechanisms.