Synthesis, characterization, cytotoxic activity and DNA binding properties of the novel dinuclear cobalt(III) complex with the condensation product of 2-acetylpyridine and malonic acid dihydrazide

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• 作者：Rabia Eshkourfu^a ; Bož ; idar &#x10c ; obelji&#x107 ; ^a ; Miroslava Vuj&#x10d ; i&#x107 ; ^b ; Iztok Turel^c ; Andrej Pevec^c ; Kristina Sep&#x10d ; i&#x107 ; ^d ; Manja Zec^e ; Siniš ; a Radulovi&#x107 ; ^e ; Tatjana Srdi&#x107 ; -Radi&#x107 ; ^e ; Dragana Miti&#x107 ; ^a ; Katarina Andjelkovi&#x107 ; ^a ; Duš ; an Sladi&#x107 ; ^a ; ^{dsladic@chem.bg.ac.rs"" rel=""nofollow}
• 关键词：Dinuclear cobalt(III) complex ; Crystal structure ; Apoptosis ; DNA binding
• 刊名：Journal of Inorganic Biochemistry
• 出版年：2011
• 出版时间：September 2011
• 年：2011
• 卷：105
• 期：9
• 页码：1196-1203
• 全文大小：936 K

文摘

A novel dinuclear cobalt(III) complex with the condensation product of 2-acetylpyridine and malonic acid dihydrazide, N′,N′²-bis[(1E)-1-(2-pyridyl)ethylidene]propanedihydrazide was synthesized and characterized by elemental analysis, spectroscopy (NMR and infrared), and X-ray crystal analysis. The complex showed a moderate activity towards Artemia salina. The highest cytotoxic potential of the complex was observed on the epithelial breast cancer (MDA-361) cell line. The investigated complex induced apoptosis, the early apoptotic cells comprising 28.18 % , compared to 5.64 % of control cells in the same phase. The interaction of the complex with calf thymus DNA (CT-DNA) was monitored by blue shift and hyperchromism in the UV–vis spectra. The observed intrinsic binding constant (K_b = 4.2 × 10⁵ M^− 1) together with structural analysis of the complex indicate the groove binding.