A sequential extraction procedure for an insight into selenium speciation in garlic

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• 作者：S ; ra Mounicou ; Mihaly Dernovics ; Katarzyna Bierla ; Joanna Szpunar
• 关键词：Selenium ; Garlic ; Sequential extraction ; SEC– ; ICP MS ; AEC– ; ICP MS ; Ion-pairing RPC– ; ICP MS
• 刊名：Talanta
• 出版年：2009
• 出版时间：15 March 2009
• 年：2009
• 卷：77
• 期：5
• 页码：1877-1882
• 全文大小：609 K

文摘

A sequential extraction procedure was developed for the fractionation of different classes of selenium species present in garlic. The consecutive steps included leaching with water, extraction of cell-wall bound species after lysis with a mixture of cellulase, chitinase and β-glucanase completed by a proteolytic attack, extraction with HCl to liberate the residual organic bound species and finally, extractions with sulfite solution and CS₂ to complete the mass balance by the recovery of Se⁰ and Se²⁻, respectively. Selenium speciation in the aqueous fractions was probed by anion-exchange and ion-pairing reversed-phase HPLC–ICP MS after purification by preparative size-exclusion LC. It was found to be strongly affected by the sample redox conditions. The peak identity was matched with a mixture of 9 compounds expected to be present in allium plants; electrospray QTOF MS turned out to be unsuccessful. Selenite, selenate and selenomethionine were the dominating species present.