A Silicate Gel for Promoting Deposition of Lipid Bilayers
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- 作者：Melzak ; Kathryn A. ; Gizeli ; Electra
- 关键词：acoustic ; bilayer ; lipids ; membranes ; supported lipid bilayer ; vesicles
- 刊名：Journal of Colloid and Interface Science
- 出版年：2002
- 出版时间：February 1, 2002
- 年：2002
- 卷：246
- 期：1
- 页码：21-28
- 全文大小：148 K
文摘
A simple method for modifying a polymer surface to induce lipid bilayer formation by vesicle fusion is described. A silicate gel was prepared by condensation of tetraethyl orthosilicate (TEOS) in the presence of acid. When applied to a poly(methylmethacrylate) substrate, either a rough or a smooth layer could be produced, depending on the method used for the application. The smooth surface induced formation of a supported lipid bilayer by fusion of lipid vesicles; the rough silicate surface induced adsorption of a vesicle layer. A high-frequency acoustic waveguide device was used to follow the initial adsorption of vesicles, the transition from a vesicle layer to a bilayer, and the formation of a complete bilayer; the time required to form a bilayer was determined as a function of lipid concentration in suspension. The presence of a bilayer on the smooth silicate surface was confirmed by fluorescence recovery after photobleaching. An additional procedure is described to modify a gold surface to induce bilayer formation.