Characterization of common SMPD1
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- 作者：Iwan Jones ; Xingxuan He ; Fourogh Katouzian ; Peter I. Darroch ; Edward H. Schuchman
- 关键词：Lysosomal storage disease ; Mutations ; Transgenic mouse
- 刊名：Molecular Genetics and Metabolism
- 出版年：2008
- 出版时间：November 2008
- 年：2008
- 卷：95
- 期：3
- 页码：152-162
- 全文大小：2371 K
文摘
Herein we describe detailed characterization of four common mutations (L302P, H421Y, R496L and ΔR608) within the acid sphingomyelinase (ASM) gene causing types A and B Niemann-Pick disease (NPD). In vitro and in situ enzyme assays revealed marked deficiencies of ASM activity in NPD cell lines homoallelic for each mutation, although Western blotting and fluorescent microscopy showed that the mutant ASM polypeptides were expressed at normal levels and trafficked to lysosomes. Co-immunoprecipitation of the polypeptides with the ER chaperone, BiP, confirmed these findings, as did in vitro expression of the mutant cDNAs in reticulocyte lysates. We further developed a computer assisted, three-dimensional model of human ASM based on homologies to known proteins, and used this model to map each NPD mutation in relation to putative substrate binding, hydrolysis and zinc-binding domains. Lastly, we generated transgenic mice expressing the R496L and ΔR608 mutations on the complete ASM knock-out background (ASMKO), and established breeding colonies for the future evaluation of enzyme enhancement therapies. Analysis of these mice demonstrated that the mutant ASM transgenes were expressed at high levels in the brain, and in the case of the ΔR608 mutation, produced residual ASM activity that was significantly above the ASMKO background.