Pneumatic cell stretching system for cardiac differentiation and culture

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• 作者：Joose Kreutzer^a ; ^c ; ¹ ; ^{joose.kreutzer@tut.fi" class="auth_mail} ; Liisa Ikonen^b ; ^c ; ¹ ; Juha Hirvonen^a ; ^c ; Mari Pekkanen-Mattila^b ; ^c ; Katriina Aalto-Set&auml ; l&auml ; ^b ; ^c ; ^d ; Pasi Kallio^a ; ^c
• 关键词：MYH6 ; alpha myosin heavy chain 6 ; MYH7 ; alpha myosin heavy chain 7 ; bFGF ; basic fibroblast growth factor ; BSA ; bovine serum albumin ; Trop I ; cardiac troponin I ; CM ; cardiomyocyte ; Cx43 ; connexin 43 ; FBS ; fetal bovine serum ; PPIG ; peptidylpropyl isomerase G (housekeeping gene) ; hESC ; human embryonic stem cell ; hiPSC ; human induced pluripotent stem cell ; hPSC ; human pluripotent stem cell ; MEF ; mouse embryonic fibroblast ; END-2 ; mouse visceral endoderm-like cell ; MCS ; multichannel systems ; NRC ; neo
• 刊名：Medical Engineering and Physics
• 出版年：April, 2014
• 年：2014
• 卷：36
• 期：4
• 页码：496-501
• 全文大小：1336 K

文摘

This paper introduces a compact mechanical stimulation device suitable for applications to study cellular mechanobiology. The pneumatically controlled device provides equiaxial strain for cells on a coated polydimethylsiloxane (PDMS) membrane and enables real time observation of cells with an inverted microscope. This study presents the implementation and operation principles of the device and characterizes membrane stretching. Different coating materials are also analyzed on an unstretched membrane to optimize the cell attachment on PDMS. As a result, gelatin coating was selected for further experiments to demonstrate the function of the device and evaluate the effect of long-term cyclic equiaxial stretching on human pluripotent stem cells (hPSCs). Cardiac differentiation was induced with mouse visceral endoderm-like (END-2) cells, either on an unstretched membrane or with mechanical stretching. In conclusion, hPSCs grew well on the stretching platform and cardiac differentiation was induced. Thus, the platform provides a new possibility to study the effect of stretching on cellular properties including differentiation and stress induced cardiac diseases.