Evaluation of the clinical sensitivity for the quantification of human immunodeficiency virus type 1 RNA in plasma: Comparison of the new COBAS TaqMan HIV-1 with three current HIV-RNA assays—LC

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• 作者：Antigoni Katsoulidou ; Maria Petrodaskalaki ; Vana Sypsa ; Eleni Papachristou ; Cleo G. Anastassopoulou ; Panagiotis Gargalianos ; Anastasia Karafoulidou ; Marios Lazanas ; Theodoros Kordossis ; Anastasia Ando
• 关键词：HIV-1 RNA ; Viral load ; Cobas TaqMan ; LCx ; Branched DNA ; COBAS Amplicor Monitor v1.5
• 刊名：Journal of Virological Methods
• 出版年：2006
• 出版时间：February 2006
• 年：2006
• 卷：131
• 期：2
• 页码：168-174
• 全文大小：256 K

文摘

The COBAS TaqMan HIV-1 test (Roche Diagnostics) was compared with the LCx HIV RNA quantitative assay (Abbott Laboratories), the Versant HIV-1 RNA 3.0 (bDNA) assay (Bayer) and the COBAS Amplicor HIV-1 Monitor v1.5 test (Roche Diagnostics), using plasma samples of various viral load levels from HIV-1-infected individuals. In the comparison of TaqMan with LCx, TaqMan identified as positive 77.5 % of the 240 samples versus 72.1 % identified by LCx assay, while their overall agreement was 94.6 % and the quantitative results of samples that were positive by both methods were strongly correlated (r = 0.91). Similarly, in the comparison of TaqMan with bDNA 3.0, both methods identified 76.3 % of the 177 samples as positive, while their overall agreement was 95.5 % and the quantitative results of samples that were positive by both methods were strongly correlated (r = 0.95). Finally, in the comparison of TaqMan with Monitor v1.5, TaqMan identified 79.5 % of the 156 samples as positive versus 80.1 % identified by Monitor v1.5, while their overall agreement was 95.5 % and the quantitative results of samples that were positive by both methods were strongly correlated (r = 0.96). In conclusion, the new COBAS TaqMan HIV-1 test showed excellent agreement with other widely used commercially available tests for the quantitation of HIV-1 viral load.