Comparative evaluation of the QUANTIPLEX HIV-1 RNA 2.0 and 3.0 (bDNA) assays and the AMPLICOR HIV-1 MONITOR v1.5 test for the quantitation of human immunodeficiency virus type 1 RNA in plasma

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• 作者：Anastassopoulou ; Cleo G. ; Touloumi ; Giota ; Katsoulidou ; Antigoni ; Hatzitheodorou ; Helen ; et. al.
• 关键词：HIV-1 RNA ; Viral load ; Branched DNA ; Amplicor
• 刊名：Journal of Virological Methods
• 出版年：2001
• 出版时间：January, 2001
• 年：2001
• 卷：91
• 期：1
• 页码：67-74
• 全文大小：88.4 K

文摘

HIV-1 RNA measurements from 84 plasma specimens obtained with the QUANTIPLEX HIV-1 RNA 2.0 and 3.0 (bDNA) assays (Chiron Diagnostics, Emeryville, CA) and with the AMPLICOR HIV-1 MONITOR™ Test, version 1.5 with ultra-sensitive specimen preparation (Roche Diagnostic Systems, Inc., Branchburg, NJ) were compared. The absolute RNA values of tested specimens differed significantly between bDNA 2.0 and bDNA 3.0 or Monitor v1.5 measurements (Wilcoxon signed-rank test P<0.001). Results generated with bDNA 3.0 or with Monitor v1.5 were approximately twofold greater than those generated with bDNA 2.0, with smaller differences at higher HIV-1 RNA levels and greater differences at RNA levels below 1000 copies per ml. Although highly correlated (r