Effect of neurotrophins on differentiation, calcification and proliferation in cultures of human pulp cells
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- 作者：Noriyoshi Mizuno ; Hideki Shiba ; Wan-peng Xu ; Takafumi Inui ; Tsuyoshi Fujita ; Mikihito Kajiya ; Katsuhiro Takeda ; Naohiko Hasegawa ; Hiroyuki Kawaguchi ; Hidemi Kurihara
- 关键词：Neurotrophins ; Human pulp cells ; Hard tissue-related proteins
- 刊名：Cell Biology International
- 出版年：2007
- 出版时间：December 2007
- 年：2007
- 卷：31
- 期：12
- 页码：1462-1469
- 全文大小：418 K
文摘
Neurotrophins (NTs) are expressed during tooth development. However, little is known about a role of NTs in differentiation of pulp cells into mineralizing cells. In this study, mRNA expressions of hard tissue-related proteins, calcification and proliferation are examined in cultures of human pulp (HP) cells. Nerve growth factor (NGF), brain derived neurotrophic factor (BDNF), neurotrophin (NT)-3 and NT-4/5 increased the mRNA levels of dentin sialophsphoprotein, alkaline phosphatase, osteopontin, type I collagen and bone morphogenetic protein-2 and mineral deposition in cultures of HP cells. The increased levels and manners varied, depending on the concentrations of NTs and hard-tissue related protein tested. On the other hand, only NGF significantly stimulated DNA synthesis in cultures of HP cells. These findings suggest that NTs characteristically regulate hard-tissue related protein expression, calcification and proliferation in pulp cells. NTs may accelerate pulp cell differentiation.