Protection against CCl₄-induced injury in liver by adenovirally introduced thioredoxin gene

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• 作者：Katsuhiro Isoda ; Eri Arita ; Midori Kojima ; Masahiro Ikkaku ; Fumi Tashiro ; Eiji Yamato ; Jun-Ichi Miyazaki ; Masaya Kawase ; Masuo Kondoh and Kiyohito Yagi
• 关键词：Thioredoxin ; Adenovirus vector ; Gene therapy ; Oxidative stress ; Liver injury
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2006
• 出版时间：10 November 2006
• 年：2006
• 卷：350
• 期：1
• 页码：157-161
• 全文大小：351 K

文摘

Antioxidation therapy is a promising strategy for treating or preventing oxidative stress-related liver diseases. The human thioredoxin (TRX) gene was inserted into an adenovirus vector (Adv-TRX), which was administered to mice. The mice were treated with 1 ml/kg CCl₄ 48 h after the infection. Blood samples were taken and the liver was excised 24 h after the CCl₄ treatment. Serum ammonia, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) levels were determined, and liver sections were stained with hematoxylin and eosin. RT-PCR analysis showed that the introduced TRX gene was expressed only in the liver. Adv-TRX decreased the serum ammonia, AST, and ALT levels. Hematoxylin-eosin staining indicated that the CCl₄-induced injury was significantly prevented by the Adv-TRX infection. The gene delivery of TRX, which plays a central role in intracellular redox control, was shown to be effective in protecting the liver against oxidative stress-induced injury.