Enhanced wound healing by topical administration of mesenchymal stem cells transfected with stromal cell-derived factor-1

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• 作者：Yoko Nakamura ; Hidefumi Ishikawa ; Katsuya Kawai ; Yasuhiko Tabata ; Shigehiko Suzuki
• 关键词：Skin wound healing ; Mesenchymal stem cells ; Stromal cell-derived factor-1 ; Non-viral vector
• 刊名：Biomaterials
• 出版年：2013
• 出版时间：December, 2013
• 年：2013
• 卷：34
• 期：37
• 页码：9393-9400
• 全文大小：2002 K

文摘

The objective of this study was to investigate the ability of mesenchymal stem cells (MSC) genetically engineered with stromal cell-derived factor-1 (SDF-1) to heal skin wounds. When transfected with SDF-1 plasmid DNA, MSC which were isolated from the bone marrow of rats, secreted SDF-1 for 7 days. In?vitro cell migration assay revealed that the SDF-1-engineered MSC (SDF-MSC) enhanced the migration of MSC and dermal fibroblasts to a significantly greater extent than MSC. The SDF-MSC secreted vascular endothelial growth factor, hepatocyte growth factor, and interleukin 6 at a significantly high level. A skin defect model of rats was prepared and MSC and SDF-MSC were applied to the wound to evaluate wound healing in terms of wound size and histological examinations. The wound size decreased significantly faster with SDF-MSC treatment than with MSC and PBS treatments. The length of the neoepithelium and the number of blood vessels newly formed were significantly larger. A cell-tracing experiment with fluorescently labeled cells demonstrated that the percent survival of SDF-MSC in the tissue treated was significantly high compared with that of MSC. It was concluded that SDF-1 genetic engineering is a promising way to promote the wound healing activity of MSC for a skin defect.