Direct assessment of plasma low density lipoprotein and high density lipoprotein cholesterol levels and coronary heart disease: Results from the Framingham Offspring Study

详细信息    查看全文

• 作者：Seiko Otokozawa ; Masumi Ai ; Bela F. Asztalos ; Charles C. White ; Serkalem Demissie-Banjaw ; L. Adrienne Cupples ; Katsuyuki Nakajima ; Peter W.F. Wilson ; Ernst J. Schaefer
• 关键词：Cholesterol ; LDL ; HDL ; Coronary heart disease ; Framingham Offspring Study
• 刊名：Atherosclerosis
• 出版年：2010
• 出版时间：November 2010
• 年：2010
• 卷：213
• 期：1
• 页码：251-255
• 全文大小：142 K

文摘

Background

We evaluated direct low density lipoprotein (LDL) cholesterol (C) and high density lipoprotein (HDL) cholesterol (C) versus standard methods using fasting plasma samples from participants in cycle 6 of the Framingham Offspring Study.

Methods

Direct LDL-C and HDL-C measurements were performed on fasting plasma from male (1335 controls, 173 CHD cases) and female (1606 controls, 74 cases) participants, and compared with LDL-C, as calculated with the Friedewald formula, and HDL-C, as measured after dextran-Mg²⁺ precipitation.

Results

Values for direct LDL-C and HDL-C correlated well with standard methods (both about r² = 0.94, p < 0.001) with similar absolute values. Biases of >10 % were present for 7.7 % of samples for LDL-C, while for HDL-C this value was 8.5 % . Despite higher use of cholesterol-lowering medication in CHD cases, calculated or direct LDL-C values were still well above recommended values [<2.6 mmol/L (100 mg/dL)] in CHD cases, especially in females.

Conclusions

Direct assays for both LDL-C and HDL-C provide an acceptable guide for lipid treatment. In Framingham Offspring Study participants most CHD cases had LDL-C levels above the recommended target.