The crystal structure of novel chondroitin lyase ODV-E66, a baculovirus envelope protein

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• 作者：Yoshirou Kawaguchi ; Nobuo Sugiura ; Koji Kimata ; Makoto Kimura ; Yoshimitsu Kakuta
• 关键词：ODV-E66 ; occlusion derived virus envelope protein 66 ; PL8 ; polysaccharide lyase 8
• 刊名：FEBS Letters
• 出版年：11 December, 2013
• 年：2013
• 卷：587
• 期：24
• 页码：3943-3948
• 全文大小：1756 K

文摘

Chondroitin lyases have been known as pathogenic bacterial enzymes that degrade chondroitin. Recently, baculovirus envelope protein ODV-E66 was identified as the first reported viral chondroitin lyase. ODV-E66 has low sequence identity with bacterial lyases at <12%, and unique characteristics reflecting the life cycle of baculovirus. To understand ODV-E66鈥檚 structural basis, the crystal structure was determined and it was found that the structural fold resembled that of polysaccharide lyase 8 proteins and that the catalytic residues were also conserved. This structure enabled discussion of the unique substrate specificity and the stability of ODV-E66 as well as the host specificity of baculovirus.