Evidence for the changes of antitumor immune response during lymph node metastasis in head and neck squamous cell carcinoma

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• 作者：Kenichi Kumagai ; Yoshiki Hamada ; Akito Gotoh ; Hiroshi Kobayashi ; Koji Kawaguchi ; Akihisa Horie ; Hiroyuki Yamada ; Satsuki Suzuki ; Ryuji Suzuki
• 刊名：Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology
• 出版年：2010
• 出版时间：September 2010
• 年：2010
• 卷：110
• 期：3
• 页码：341-350
• 全文大小：1813 K

文摘

Objective

This study aimed to elucidate the differences in antitumor immune responses between primary tumors and metastatic regional lymph nodes in head and neck squamous cell carcinoma (HNSCC).

Study design

The clonality of tumor-infiltrating lymphocytes in tissue specimens from 17 HNSCC patients was examined regarding their T-cell receptor (TCR) repertoires and their complementary determining region 3 (CDR3) size spectratyping. Cytokine expression profiles and T-cell phenotypes also were measured by using real-time quantitative polymerase chain reaction.

Results

The host immune responses to HNSCC cells, reflected by the TCR repertoire, differed between primary tumors and metastatic lymph nodes. CD8⁺-T cells and T helper type 1 (T_H1)/T cytotoxic 1 (T_C1) cell cytokine production in metastatic and nonmetastatic lymph nodes were similar.

Conclusions

The antitumor immune response to HNSCC cells changes during lymph node metastasis, and HNSCC cells can escape the cytotoxic immune responses mediated by CD8⁺-T cells and T_H1/T_C1 cells. These results suggest that lymph node metastasis might be associated with changes in the nature of the primary tumor antigens.