RNase P plays essential role in non-tRNA biogenesis in bacterial and eukaryotic cells.
The mutant cell grew slowly compared with wild-type Thermococcus kodakarensis.
RNase P isolated from the mutant cell had reduced pre-tRNA cleavage activity.
The mutant cell accumulated unprocessed transcripts for pre-tRNAs.
The result demonstrates the preparation of the archaeal mutant with impaired RNase P.