Reduction of chlortetracycline-resistant Escherichia coli

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• 作者：Yuri Kobashi ; Hideyuki Ohmori ; Kiyoshi Tajima ; Tomoyuki Kawashima ; Hiroo Uchiyama
• 关键词：Fermented liquid feed (FLF) ; Weaned piglet ; Lactic acid bacteria ; Chlortetracycline resistant ; Tetracycline-resistance gene
• 刊名：Anaerobe
• 出版年：2008
• 出版时间：October 2008
• 年：2008
• 卷：14
• 期：4
• 页码：201-204
• 全文大小：244 K

文摘

We investigated the change in chlortetracycline resistance in 360 Escherichia coli strains separated from the feces of piglets fed fermented liquid feed (FLF) in comparison with those fed dry feed (control). The total amount of lactic acid bacteria in feces was 8.77×10⁸ CFU/g DM at weaning, which increased to 1.23×10¹² CFU/g DM (FLF) at 28 days after weaning (P<0.001). As a result of the antibiotic susceptibility test, almost all isolates were chlortetracycline-resistant (CTC^R) until 14 days after weaning both in the FLF and control groups. At 28 days, the CTC^R E. coli decreased to 22.2 % in the FLF group, while the proportion of resistant bacteria was 88.9 % in the control group. In addition, as a result of the gene analysis, it was clarified that there is a relation between the decrease in the minimum inhibitory concentration values and the possession rate of the tetracycline-resistance gene tet(A), tet(B) and tet(D). These results show that FLF caused an increase in the number of lactic acid bacteria in the intestines, and suggested that the feeding of FLF can possibly reduce antibiotic-resistance bacteria.