Short-lived fluorescence component of DPH reports on lipid–water interface of biological membranes

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• 作者：Konopá ; sek ; Ivo ; Vecer ; Jaroslav ; Strzalka ; Kazimierz ; Amler ; Evzen
• 关键词：Diphenylhexatriene ; Fluorescence lifetime ; Lipid phase transition ; Alcohol
• 刊名：Chemistry and Physics of Lipids
• 出版年：2004
• 出版时间：July, 2004
• 年：2004
• 卷：130
• 期：2
• 页码：135-144
• 全文大小：137 K

文摘

Fluorescence measurements of 1,6-diphenyl-1,3,5-hexatriene (DPH) in large unilamellar phospholipid vesicles were performed to characterize the influence of the membrane physical properties on the short-lived lifetime component of the fluorescence decay. We have found that the short-lived component of DPH significantly shortens when the membrane undergoes a temperature-induced phase transition as it is known for the long-lived component of DPH. We induced membrane phase transitions also by alcohols, which are reported to be distributed different way in the membrane–ethanol close to the membrane-water interface and benzyl alcohol in the membrane core. A different effect of the respective alcohol on the short and long decay component was observed. Both the time-resolved fluorescence spectra of DPH taken during lipid vesicle staining and the lifetime dependences caused by changes of temperature and/or induced by the alcohols show that the short-lived fluorescence originates from the population of dye molecules distributed at the membrane–water interface.