Expression patterns of the Egr1 and Egr3 genes during medaka embryonic development

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• 作者：Tomonori Deguchi ; Kazuhiro E. Fujimori ; Takashi Kawasaki ; Liao Xianghai ; Shunsuke Yuba
• 关键词：Egr1 ; zif268 ; NGFI-A ; Krox24 ; Tis8 ; ZENK ; Egr3 ; Krox20 ; Medaka ; Telencephalon ; Diencephalon ; Hypothalamus ; Retina ; Optic tectum ; Torus semicircularis ; Rhombencephalon ; Heart ; Pharynx ; Pectoral fin
• 刊名：Gene Expression Patterns
• 出版年：2009
• 出版时间：April 2009
• 年：2009
• 卷：9
• 期：4
• 页码：209-214
• 全文大小：1042 K

文摘

Egr1 and Egr3 are zinc finger-type transcription factors and known as synaptic activity-inducible immediate-early genes. Egr1 also plays important roles in many aspects of vertebrate development. Egr3 is known as the gene that is related to biological rhythm and muscular development, but its behavior in the central nervous system during development is not clear. We cloned the cDNA of the egr1 and egr3 orthologs in medaka, and examined their expression patterns during embryonic development using whole-mount in situ hybridization. Medaka egr3 was the first cloned egr3 gene in fish. The expression of egr1 mRNA was first detected at 1 day post-fertilization (dpf). It was expressed in the whole embryonic body. At 3 dpf, the egr1 mRNA was strongly expressed in the telencephalon, diencephalon, hypothalamus, optic tectum, dorsal medulla oblongata, retina, heart, pharynx, and pectoral fin. The expression of egr3 mRNA was first detected at 3 dpf. It was expressed in the telencephalon, hypothalamus, optic tectum, and pharynx. By sectioning the whole-mount specimens, expression of both the egr1 and egr3 mRNAs were observed in the telencephalon, hypothalamus, and optic tectum. However, the positions at which the genes were expressed were different.