Quantification of fat-soluble vitamins in human breast milk by liquid chromatography–tandem mass spectrometry

详细信息    查看全文

• 作者：Maya Kamao ; Naoko Tsugawa ; Yoshitomo Suhara ; Akimori Wada ; Toshiyuki Mori ; Kazuo Murata ; Riichiro Nishino ; Tetsuya Ukita ; Kazuhiro Uenishi ; Kiyoshi Tanaka ; Toshio Okano
• 关键词：Vitamin A ; Vitamin D ; Vitamin E ; Vitamin K ; Fat-soluble vitamins ; Liquid chromatography– ; tandem mass spectrometry ; Breast milk
• 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
• 出版年：2007
• 出版时间：15 November 2007
• 年：2007
• 卷：859
• 期：2
• 页码：192-200
• 全文大小：264 K

文摘

Sensitive quantification method for fat-soluble vitamins in human breast milk by liquid chromatography–tandem mass spectrometry was developed. Vitamins A, D and E were extracted from 10.0 mL of breast milk after saponifying by basic condition. Vitamin K derivatives were extracted from 3.0 mL of breast milk after lipase treatment. The corresponding stable isotope-labeled compounds were used as internal standards. For the determination of vitamin D compounds, derivatization with a Cookson-type reagent was performed. All fat-soluble vitamins were determined by liquid chromatography–tandem mass spectrometry in the positive ion mode. The detection limits of all analytes were 1–250 pg per 50 μL. The recoveries of fat-soluble vitamins were 91–105 % . Inter-assay CV values of each vitamin were 1.9–11.9 % . The mean concentrations of retinol, vitamin D₃, 25-hydroxyvitamin D₃, α-tocopherol, phylloquinone and menaquinone-4 were 0.455 μg/mL, 0.088 ng/mL, 0.081 ng/mL, 5.087 μg/mL, 3.771 ng/mL, and 1.795 ng/mL, respectively (n = 82). This method makes possible to determine fat-soluble vitamins with a wide range of polarities in human breast milk. The assay may be useful for large-scale studies.