Recombinant cellulolytic or xylanolytic complex comprising the full-length scaffolding protein RjCipA and cellulase RjCel5B or xylanase RjXyn10C of Ruminiclostridium josui
Ruminiclostridium josui scaffolding protein (RjCipA) was expressed by E. coli. RjCel5B and RjXyn10C are cellulosomal endoglucanase and endoxylanase, respectively. RjCel5B-RjCipA complex showed a strong substrate-targeting effect toward rice straw. RjXyn10C-RjCipA complex showed a substrate-isolating effect toward rice straw. Proximity effect by adjoining enzymes was observed in neither of the complexes.