Localization and Changes of Diamine Oxidase During Cardiopulmonary Bypass in Rabbits

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• 作者：Nobuo Tsunooka ; Kazutaka Maeyama ; Hiromichi Nakagawa ; Takashi Doi ; Atsushi Horiuchi ; Katsutoshi Miyauchi ; Yuji Watanabe ; Hiroshi Imagawa and Kanji Kawachi
• 刊名：Journal of Surgical Research
• 出版年：2006
• 出版时间：March 2006
• 年：2006
• 卷：131
• 期：1
• 页码：58-63
• 全文大小：218 K

文摘

Background

We previously observed increased serum diamine oxidase activity during clinical cardiopulmonary bypass, indicating small intestinal mucosal ischemia followed by bacterial translocation.

Materials and methods

In seven female rabbits undergoing cardiopulmonary bypass for 1 h, we analyzed the localization of diamine oxidase immunohistochemically, and measured its activity in serum and abdominal organs before and after cardiopulmonary bypass (CPB).

Results

Preoperatively, diamine oxidase activity and immunoreactivity were high in the small intestine, localized to villus tips. Serum activity increased significantly after CPB, whereas small intestinal diamine oxidase decreased with mucosal injury.

Conclusions

In this model serum diamine oxidase activity appeared to reflect CPB induced intestinal mucosal injury.