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• 作者：Kataoka ; Tatsuki R. ; Morii ; Eiichi ; Oboki ; Keisuke ; Jippo ; Tomoko ; Maeyama ; Kazutaka ; Kitamura ; Yukihiko
• 关键词：MITF ; Mast cell ; Mutant mice ; Transcription factor
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2002
• 出版时间：September 13, 2002
• 年：2002
• 卷：297
• 期：1
• 页码：111-115
• 全文大小：178 K

文摘

MITF is a basic helix-loop-helix leucine zipper-type transcription factor and is important for development of mast cells. MITF encoded by Mi^wh allele (Mi^wh-MITF) was mutated at a single amino acid of basic domain, and possessed a deficient but apparent DNA-binding ability. Here, we characterized the unique effects of Mi^wh-MITF on the expression of mast cell-related genes. The expression level of mouse mast cell protease (mMCP)-4, -5, and -6 genes in Mi^wh/Mi^wh cultured mast cells (CMCs) was intermediate between levels of normal (+/+) CMCs and tg/tg CMCs, which did not express any MITFs. Mi^wh-MITF appeared to show the positive transactivation effect through the remaining DNA-binding ability. On the other hand, the expression level of tryptophan hydroxylase gene was lower in Mi^wh/Mi^wh CMCs than in tg/tg CMCs, suggesting the inhibitory effect of Mi^wh-MITF on the transactivation. Mi^wh-MITF possessed dual abnormal effects on transactivation of mast cell-related genes.