Mechanism of Ubiquitination and Deubiquitination in the Fanconi Anemia Pathway

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• 作者：Sylvie van Twest¹ ; Vincent J. Murphy¹ ; Charlotte Hodson¹ ; Winnie Tan¹ ; ⁴ ; Paolo Swuec³ ; Julienne J. O&rsquo ; Rourke¹ ; ⁴ ; Jö ; rg Heierhorst² ; ⁴ ; Wayne Crismani¹ ; Andrew J. Deans¹ ; ⁴ ; ⁵ ; ^{adeans@svi.edu.au}
• 关键词：Fanconi anemia ; core complex ; monoubiquitination ; RING E3 ; deubiquitination ; FANCD2 ; FANCB ; DNA repair ; enzyme mechanism
• 刊名：Molecular Cell
• 出版年：2017
• 出版时间：19 January 2017
• 年：2017
• 卷：65
• 期：2
• 页码：247-259
• 全文大小：3225 K
• 卷排序：65

文摘

Reconstitution of the Fanconi anemia (FA) pathway using recombinant proteins FANCB dimer coordinates FANCD2:FANCI monoubiquitination by two FANCL RING-ligases FANCC and FANCE provide FANCL specificity toward DNA-bound FANCD2:FANCI dimers Deubiquitination of FANCD2:FANCI by USP1:UAF1 occurs only when DNA is removed