Profiling adrenal 11β-hydroxyandrostenedione metabolites in prostate cancer cells, tissue and plasma: UPC²-MS/MS quantification of 11β-hydroxytestosterone, 11keto-testosterone and 11keto-dihydrotestosterone

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• 作者：Therina du Toit^a ; Liezl M. Bloem^a ; Jonathan L. Quanson^a ; Riaan Ehlers^a ; Antonio M. Serafin^b ; Amanda C. Swart^a ; ^{acswart@sun.ac.za}
• 关键词：Castration-resistant prostate cancer (CRPC) ; Adrenal 11β -hydroxy-androstenedione (11β OHA4) ; LNCaP prostate cancer cell model ; PNT2 epithelial prostate cell model ; 11keto-testosterone (11KT) ; LC&ndash ; MS/MS ; 11K-DHT
• 刊名：The Journal of Steroid Biochemistry and Molecular Biology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：166
• 期：Complete
• 页码：54-67
• 全文大小：1931 K
• 卷排序：166

文摘

UPC2-MS/MS show alternative pathway is dominant in PNT2 prostate cells. 11OHA4 metabolism in PNT2 cells is less efficient than in LNCaP cells. 11KDHT is efficiently glucuronidated in LNCaP cells while 11KT conjugation is poor. In PCa tissue 11OHA4, 11KT, 11OHT and 11KDHT are present at significant levels. In PCa plasma 11OHA4, 11KT and 11KDHT are significantly higher than A4, T and DHT.