The eukaryotic genome, its reads, and the unfinished assembly

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• 作者：Jos¨¦ Fern ; o Mu?oz ; Juan Esteban Gallo ; Elizabeth Misas ; Juan Guillermo McEwen ; Oliver Keatinge Clay
• 关键词：NGS ; next generation sequencing ; GC ; guanine and cytosine level
• 刊名：FEBS Letters
• 出版年：2013
• 出版时间：11 July, 2013
• 年：2013
• 卷：587
• 期：14
• 页码：2090-2093
• 全文大小：301 K

文摘

In recent years, readily affordable short read sequences provided by next-generation sequencing (NGS) have become longer and more accurate. This has led to a jump in interest in the utility of NGS-only approaches for exploring eukaryotic genomes. The concept of a static, ¡®finished¡¯ genome assembly, which still appears to be a faraway goal for many eukaryotes, is yielding to new paradigms. We here motivate an object-view concept where the raw reads are the main, fixed object, and assemblies with their annotations take a role of dynamically changing and modifiable views of that object.