- 作者：Yoichi Miura ; MD ; PhD* ; yoimiu@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Hiroshi Tanemura ; MD ; PhD* ; Masashi Fujimoto ; MD ; PhD* ; Kazuhide Hamada ; MD ; PhD* ; Keiichi Miyamoto ; PhD&dagger ; ; Naoki Toma ; MD ; PhD* ; Kyoko Imanaka-Yoshida ; MD ; PhD&Dagger ; ; Satoshi Matsushima ; MD ; PhD* ; Toshimichi Yoshida ; MD ; PhD&Dagger ; ; Waro Taki ; MD ; PhD* ; Hidenori Suzuki ; MD ; PhD*
- 关键词：Tenascin-C ; gellan sulfate ; transforming growth factor-beta ; elastase-induced rabbit aneurysms ; coil embolization
- 刊名：Journal of Stroke and Cerebrovascular Diseases
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：25
- 期：4
- 页码：771-780
- 全文大小：2403 K
Methods
Elastase-induced rabbit side-wall aneurysms were randomly coiled via a transfemoral route like clinical settings with platinum coils (PCs), gellan sulfate core platinum coils (GSCCs), or GSCC-TNCs (n = 5, respectively). Aneurysm-occlusion status was evaluated angiographically and histologically at 2 weeks post coiling. As each rabbit coiled aneurysm provided only 2-3 tissue slices due to technical limitations and prevented immunohistochemical evaluations, a PC, GSCC, or GSCC-TNC was randomly implanted in a rat blind-ended model (n = 3, respectively) and the organization effects were immunohistochemically evaluated for expressions of tenascin-C (TNC), transforming growth factor-beta (TGF-β), and matrix metalloproteinase-9 (MMP-9) 2 weeks later.
Results
Coil handling was similar among the 3 kinds of coils. GSCCs showed a significantly higher ratio of organized area to the aneurysmal cavity than PCs, but GSCC-TNCs had the greatest organization-promoting effects on aneurysms (the ratio of organized area/aneurysmal luminal area: PC, 17.9 ± 7.1%; GSCC, 54.2 ± 18.3%; GSCC-TNC, 82.5 ± 5.8%). GSCC-TNCs had intense immunoreactivities for TNC, TGF-β, and MMP-9 in the organized thrombosis and tunica media. GSCCs also showed intense immunoreactivities for TNC, TGF-β, and MMP-9, although the extent was less than GSCC-TNCs. The immunoreactivities were hardly found in unorganized thrombus and the tunica media of aneurysm wall in the PC group.
Conclusions
This study first showed that GSCC-TNCs promote intra-aneurysmal clot organization in simulated clinical settings using rabbits possibly through the TGF-β and MMP-9 upregulation.