Cooperative expression of survival p-ERK and p-Akt signals in rat brain neurons after transient MCAO

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• 作者：Li ; Feng ; Omori ; Nobuhiko ; Jin ; Guang ; Wang ; Shao Jun ; Sato ; Keiko ; Nagano ; Isao ; Shoji ; Mikio ; et. al.
• 关键词：Disorders of the nervous system ; Ischemia ; CBF ; cerebral blood flow ; DP ; double positive ; ERK ; extracellular signal-regulated kinase ; FITC ; fluorescein-isothiocyanate ; IC ; ischemic core ; IP ; ischemic penumbra ; MCA ; middle cerebral artery ; MCAO ; middle cer
• 刊名：Brain Research
• 出版年：2003
• 出版时间：February 7, 2003
• 年：2003
• 卷：962
• 期：1-2
• 页码：21-26
• 全文大小：136 K

文摘

In order to determine possible coordinate expression of major survival signals, immunofluorescent analyses for phosphorylated ERK (p-ERK) and phosphorylated Akt (p-Akt) were carried out after 90 min of transient middle cerebral artery occlusion (MCAO) in rats. p-Akt single positive cells (E−/A+) were found in the sham control brains with weak signal intensity. The levels of both survival signals concurrently increased from 1 to 3 h after the reperfusion with the peak at 1 h, and the signals were much stronger in the ischemic penumbra (IP) than ischemic core (IC). The number of E−/A+ cells was larger in both the IC and IP than that of p-ERK single positive cells (E+/A−). The E+/A− cells were primarily expressed at 1 h in the IP. The number of p-ERK plus p-Akt double positive cells (E+/A+) peaked at 1 h, and the intensity was much stronger in the IP than IC. These findings suggest that p-ERK and p-Akt play independent roles, respectively as emergency or maintenance signal for survival at an early stage after reperfusion, and that both signals were cooperatively expressed especially in the IP.