SAW1 is required for SDSA double-strand break repair in S. cerevisiae

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• 作者：Graciel Diamante¹ ; Claire Phan¹ ; Angie S. Celis ; Jonas Krueger ; Eric P. Kelson ; Paula L. Fischhaber ; ^{paula.fischhaber@csun.edu" class="auth_mail}
• 关键词：Saw1 ; Rad10 ; Double strand break repair ; Synthesis Dependent Strand Annealing
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：14 March, 2014
• 年：2014
• 卷：445
• 期：3
• 页码：602-607
• 全文大小：742 K

文摘

SAW1, coding for Saw1, is required for single-strand annealing (SSA) DNA double-strand break (DSB) repair in Saccharomyces cerevisiae. Saw1 physically associates with Rad1 and Rad52 and recruits the Rad1-Rad10 endonuclease. Herein we show by fluorescence microscopy that SAW1 is similarly required for recruitment of Rad10 to sites of Synthesis-Dependent Strand Annealing (SDSA) and associates with sites of SDSA repair in a manner temporally overlapped with Rad10. The magnitude of induction of colocalized Saw1-CFP/Rad10-YFP/DSB-RFP foci in SDSA is more dramatic in S and G2 phase cells than in M phase, consistent with the known mechanism of SDSA. We observed a substantial fraction of foci in which Rad10 was localized to the repair site without Saw1, but few DSB sites that contained Saw1 without Rad10. Together these data are consistent with a model in which Saw1 recruits Rad1-Rad10 to SDSA sites, possibly even binding as a protein-protein complex, but departs the repair site in advance of Rad1-Rad10.