- 作者：Kwok-Hung Chan ; Jasper Fuk-Woo Chan ; Herman Tse ; Honglin Chen ; C ; y Choi-Yi Lau ; Jian-Piao Cai ; Alan Ka-Lun Tsang ; Xincai Xiao ; Kelvin Kai-Wang To ; Susanna Kar-Pui Lau ; Patrick Chiu-Yat Woo ; Bo-Jiang Zheng ; Ming Wang ; Kwok-Yung Yuen
- 关键词：Coronavirus ; Betacoronavirus ; EMC ; SARS ; OC43 ; HKU1 ; Cross-reactive ; Antibody ; Neutralization ; Seroprevalence
- 刊名：Journal of Infection
- 出版年：2013
- 出版时间：August, 2013
- 年：2013
- 卷：67
- 期：2
- 页码：130-140
- 全文大小：2553 K
Summary
Objectives
A severe acute respiratory syndrome (SARS)-like disease due to a novel betacoronavirus, human coronavirus EMC (HCoV-EMC), has emerged recently. HCoV-EMC is phylogenetically closely related to Tylonycteris-bat-coronavirus-HKU4 and Pipistrellus-bat-coronavirus-HKU5 in Hong Kong. We conducted a seroprevalence study on archived sera from 94 game-food animal handlers at a wild life market, 28 SARS patients, and 152 healthy blood donors in Southern China to assess the zoonotic potential and evidence for intrusion of HCoV-EMC and related viruses into humans.Methods
Anti-HCoV-EMC and anti-SARS-CoV antibodies were detected using screening indirect immunofluorescence (IF) and confirmatory neutralizing antibody tests.
Results
Two (2.1 % ) animal handlers had IF antibody titer of ¡Ý1:20 against both HCoV-EMC and SARS-CoV with neutralizing antibody titer of <1:10. No blood donor had antibody against either virus. Surprisingly, 17/28 (60.7 % ) of SARS patients had significant IF antibody titers with 7/28 (25 % ) having anti-HCoV-EMC neutralizing antibodies at low titers which significantly correlated with that of HCoV-OC43. Bioinformatics analysis demonstrated a significant B-cell epitope overlapping the heptad repeat-2 region of Spike protein. Virulence of SARS-CoV over other betacoronaviruses may boost cross-reactive neutralizing antibodies against other betacoronaviruses.
Conclusions
Convalescent SARS sera may contain cross-reactive antibodies against other betacoronaviruses and confound seroprevalence study for HCoV-EMC.