A mechanistic investigation on anticholinesterase and antioxidant effects of rose (Rosa damascena Mill.)
详细信息 查看全文
- 作者：Fatma Sezer Senol ; Ilkay Erdogan Orhan ; Mine Kurkcuoglu ; Mahmud Tareq Hassan Khan ; Ayten Altintas ; Bilge Sener ; Kemal Husnu Can Baser
- 关键词：Rose ; Cholinesterase inhibition ; Antioxidant activity ; Molecular docking ; Phenylethyl alcohol
- 刊名：Food Research International
- 出版年：2013
- 出版时间：August, 2013
- 年：2013
- 卷：53
- 期：1
- 页码：502-509
- 全文大小：470 K
文摘
In the current study, neuroprotective effect of the essential oil and aromatic waters of rose was investigated by in vitro and in silico methods. The samples were tested for their inhibitory activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Since oxidative damage is associated with neurodegeneration, antioxidant activity of the samples was determined by DPPH radical scavenging, metal-chelation, and ferric-reducing antioxidant power (FRAP) assays. Their chemical composition was elucidated by GC-MS. Anti-cholinesterase effects of the main components (citronellol, geraniol, nerol, and phenylethyl alcohol) were also examined. The rose oil showed a noteworthy inhibition against AChE (60.86 ¡À 1.99 % ) and BChE (51.08 ¡À 1.70 % ) at 1000 ¦Ìg/mL and low activity in DPPH radical scavenging and FRAP tests. Phenylethyl alcohol exerted higher cholinesterase inhibition than other components and applied further to molecular docking simulations. Docking and binding energies supporting experimental results show that the compound is more selective towards BChE than AChE.