• 作者：Mariangela Longini ; Serafina Perrone ; Piero Vezzosi ; Barbara Marzocchi ; Antonio Kenanidis ; Giovanni Centini ; Lucia Rosignoli ; Giuseppe Buonocore
• 关键词：Oxidative stress ; Lipid peroxidation ; Reactive oxygen species ; Amniotic fluid ; Chorioamniotic sac ; High risk pregnancies
• 刊名：Clinical Biochemistry
• 出版年：2007
• 出版时间：July 2007
• 年：2007
• 卷：40
• 期：11
• 页码：793-797
• 全文大小：195 K

Premature rupture of membranes (PROM) is caused by collagen damage in the chorioamniotic sac leading to tearing. Reactive oxygen species (ROS) may be the cause of collagen damage. Isoprostanes (F₂-IP) are produced by ROS attack on polyunsaturated fatty acids and are sensitive and specific biomarkers of lipid-peroxidation in vivo.

Aim:

To verify whether oxidative stress occurs in pregnancies associated with preterm PROM.

Methods:

F₂-IPs were measured in amniotic fluid of 16 pregnancies with preterm PROM (Group II) and 97 without PROM (Group I).

Results:

F₂-IP concentrations (pg/mL) were significantly higher in group II than group I (p < 0.0001). The ROC curve showed a sensitivity of 100 % and a specificity of 84.5 % at a cut-off of 124.4 pg/mL.

Conclusions:

An association exists between oxidative stress in pregnancy and preterm PROM. The detection of amniotic fluid F₂-IP concentrations seems to be a reliable predictive index of risk of preterm PROM.