Rapid and sensitive detection of Little cherry virus 2 using isothermal reverse transcription-recombinase polymerase amplification
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- 作者：Tefera A. Mekuriaa ; 1 ; Shulu Zhangb ; Kenneth C. Eastwella ; keastwell@wsu.edu" class="auth_mail
- 关键词：Recombinase polymerase amplification ; Little cherry virus 2 ; Sweet cherry ; Prunus avium ; Little cherry disease ; Isothermal detection
- 刊名：Journal of Virological Methods
- 出版年：1 September 2014
- 年：2014
- 卷：205
- 期：Complete
- 页码：24-30
- 全文大小：2948 K
文摘
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A reverse transcriptase recombinase polymerase amplification assay (RT-RPA) was developed for the detection of Little cherry virus 2 (LChV-2).
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Detection with RT-RPA is simple, fast, cost effective and as sensitive as RT-PCR.
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Incubation for the RT-RPA is performed at a single temperature (39 °C) for only 15 min before visual detection of end point amplicons.
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Amplification with RT-RPA is performed on crude extracts of plant samples.