Our study of Rugoglobigerina specimens recovered at three mid-low latitude localities (Exmouth Plateau, eastern Indian Ocean; Shatsky Rise, northwestern Pacific Ocean; Eratosthenes Seamount, eastern Mediterranean) confirms that meridional ornamentation is a primary character of their tests, but development of this feature is highly variable throughout the geographic and stratigraphic distribution of the genus. Within assemblages of Rugoglobigerina, there is a continuous morphological range from specimens with well-developed costellae arranged in a meridional pattern to specimens with relatively short ridges that are randomly oriented to meridionally aligned. Stable isotope (未13C and 未18O) analyses indicate that specimens showing a more strongly developed meridional ornamentation consistently yield higher 未13C values than co-occurring less ornamented morphotypes at each examined locality, whereas patterns in the 未18O values are site-dependent. Interpretation of these patterns is not simple and might be related to different controlling factors acting together or separately. Potential explanations for the differential development of the ornamentation include: (1) adaptation to different ecological niches within a population, (2) species-level genetic differences, (3) variation in metabolic rate or activity within individuals, and (4) changes in the seawater carbonate ion saturation through space and time, including seasonality. In addition, we highlight the difficulty of discriminating between Rugoglobigerina and Costellagerina when they co-occur in lower Campanian deposits and among Rugoglobigerina, Costellagerina and Archaeoglobigerina at high latitude localities where species placed in all three genera show very similar shell morphology and ornamentation. Finally, our study challenges the general assumption that some external features of the wall are sufficiently reliable for taxonomic discrimination of Cretaceous planktonic foraminifera. This latter conclusion suggests that environmental conditions such as water temperature, water column stratification, nutrient concentration and carbonate ion saturation may have played a key role in triggering the external expression of genetically controlled wall ornamentation in the planktonic foraminifera.