Evaluation of loading characteristics and IgG binding performance of Staphylococcal protein A on polypropylene capillary-channeled polymer fibers
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- 作者：Hung K. Trang ; Abby J. Schadock-Hewitt1 ; Liuwei Jiang ; R. Kenneth Marcus ; marcusr@clemson.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Protein A ; IgG ; Capillary-channeled polymer ; Fibers ; Downstream processing
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：15 March 2016
- 年：2016
- 卷：1015-1016
- 期：Complete
- 页码：92-104
- 全文大小：1948 K
文摘
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Surfaces of polypropylene (PP) C-CP fibers are modified with recombinant protein A (rSPA) through an adsorption process.
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Binding under dynamic conditions (28 mm s−1) follows a Langmuir-linear isotherm form.
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Surface modification does not degrade column permeability nor the mass transfer properties of proteins.
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Dynamic loading of IgG is very rapid.
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Clean-in-place processes (urea, guanidine HCl, and NaOH) show relatively little degradation in overall efficiency over 10 cycles.