Direct isopropanol production from cellobiose by engineered Escherichia coli using a synthetic pathway and a cell surface display system

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• 作者：Yuki Soma ; Kentaro Inokuma ; Tsutomu Tanaka ; Chiaki Ogino ; Akihiko Kondo ; Masahiro Okamoto ; Taizo Hanai
• 关键词：Synthetic pathway ; Cell surface display system ; Isopropanol ; Cellobiose ; Escherichia coli
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2012
• 出版时间：July, 2012
• 年：2012
• 卷：114
• 期：1
• 页码：80-85
• 全文大小：307 K

文摘

Efficient bio-production from lignocellulosic biomass is required for the purpose of developing an inexpensive, practical bio-refinery process. As one approach to address this problem, we genetically engineered Escherichia coli to produce isopropanol directly from cellobiose via the cellobiose degradation by Beta-Glucosidase (BGL) on the cell surface. First, we investigated the cellobiose consumption of two E.?coli strains with the BGL protein from Thermobifida fusca YX (Tfu0937) fused to the anchor protein Blc (Tfu0937/Blc) using different fusion sites. Next, we introduced the synthetic pathway for isopropanol production into those strains and compared their isopropanol production in the presence of glucose. Based on the results of these assays, TA212/pTA411, which was introduced Tfu-Blc fused protein expression system and the synthetic pathway for isopropanol production, was selected for the directly isopropanol production from cellobiose. TA212/pTA411 produced 69.0 ¡À 11.6 mM isopropanol at 21 h of fermentation, whereas TA212/pTA147, which did not introduced the BGL/anchor fused protein but was introduced the synthetic pathway for isopropanol production, showed no cellobiose consumption and no isopropanol production during fermentation. To our knowledge, this is the first report of the production of a bio-product from cellobiose using E.?coli.