Regioselective synthesis of mannobiose and mannotriose by reverse hydrolysis using a novel 1,6-α-d-mannosidase from Aspergillus phoenicis
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- 作者：Athanasopoulos ; Vasileios I. ; Niranjan ; Keshavan ; Rastall ; Robert A.
- 关键词：Mannobiose ; Mannotriose ; Oligosaccharide synthesis ; 1 ; 6-α ; -d-Mannosidase ; Aspergillus phoenicis
- 刊名：Journal of Molecular Catalysis B: Enzymatic
- 出版年：2004
- 出版时间：March, 2004
- 年：2004
- 卷：27
- 期：4-6
- 页码：215-219
- 全文大小：110 K
文摘
A novel 1,6-α-d-mannosidase was produced by Aspergillus phoenicis grown on a commercial manno-oligosaccharide preparation in liquid culture. The enzyme hydrolysed only α-d-Manp-(1→6)-d-Manp and did not act on α-d-Manp-(1→2)-d-Manp, or α-d-Manp-(1→3)-d-Manp. The 1,6-α-d-mannosidase was used for synthesis of manno-oligosaccharides by reverse hydrolysis reaction. The highest yields, expressed as percentages (w/w) of total sugar, were 
21 % mannobiose and 5 % mannotriose, and they were obtained with 45 % (w/w) initial mannose concentration at pH 4.5 after 12 days incubation at 55°C. The disaccharide and trisaccharide products were separated and their structures determined by methylation analysis. Only 1–6 linkages were found in both of them.
21 % mannobiose and 5 % mannotriose, and they were obtained with 45 % (w/w) initial mannose concentration at pH 4.5 after 12 days incubation at 55°C. The disaccharide and trisaccharide products were separated and their structures determined by methylation analysis. Only 1–6 linkages were found in both of them.