Thermal and high hydrostatic pressure inactivation of myrosinase from green cabbage: A kinetic study

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• 作者：Sameer Khalil  ; Ghawi  ; ;   ; ^{Ghawi_78@yahoo.com} ; Lisa  ; Methven ; Robert A  ; Rastall ; Keshavan  ; Niranjan
• 关键词：Brassica ; Myrosinase ; Processing ; Modelling ; Stability
• 刊名：Food Chemistry
• 出版年：2012
• 出版时间：15 April 2012
• 年：2012
• 卷：131
• 期：4
• 页码：1240-1247
• 全文大小：553 K

文摘

Myrosinase, a family of enzymes which coexist with glucosinolates in all Brassica vegetables, catalyses the hydrolysis of glucosinolates to yield compounds that can have beneficial effects on human health. In this study, the thermal and pressure inactivation of myrosinase from green cabbage was kinetically investigated. Thermal inactivation started at 35 ¡ãC and inactivation kinetics was studied in the temperature range 35?5 ¡ãC. Thermal inactivation of green cabbage myrosinase followed the well known consecutive step model. Pressure inactivation started at 300 MPa, even at 10 ¡ãC, and the consecutive step model effectively described pressure inactivation in the range 300?50 MPa at 10 ¡ãC. The combined effects of applying various pressures and temperatures on myrosinase inactivation kinetics were studied in the ranges 35?0 ¡ãC and, 100?00 MPa. The inactivation followed first-order kinetics at all of the applied combinations. This study demonstrates that myrosinase from green cabbage is highly susceptible to both thermal and high pressure processing. Furthermore, it is also noted that myrosinase stability during processing appears to vary widely between different Brassica species.