Fibroblast functionality on novel Ti30Ta nanotube array

详细信息    查看全文

• 作者：Patricia Capellato ; Barbara S. Smith ; Ketul C. Popat ; Ana P.R. Alves Claro
• 关键词：Anodization ; Ti30Ta nanotubes ; Human dermal fibroblasts
• 刊名：Materials Science and Engineering: C
• 出版年：2012
• 出版时间：1 October, 2012
• 年：2012
• 卷：32
• 期：7
• 页码：2060-2067
• 全文大小：1535 K

文摘

In this study, the mechanical substrate and topographical surface properties of anodized Ti30Ta alloy were investigated using scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS) and contact angle measurement. The anodization process was performed in an electrolyte solution containing HF (48 % ) and H₂SO₄ (98 % ) in the volumetric ratios 1:9 with the addition of 5 % dimethyl sulfoxide (DMSO) at 15 V, 25 V and 35 V for 20 and 40 min, producing a nanotube architecture when anodized at 35 V for 40 min. Human dermal fibroblasts (HDF, neonatal) were utilized to evaluate the biocompatibility of Ti30Ta nanotubes and Ti30Ta alloy after 1 and 3 days of culture. Cellular adhesion, proliferation, viability, cytoskeletal organization and morphology were investigated using fluorescence microscope imaging, biochemical assay and SEM imaging respectively. The results presented identify altered material properties and improved cellular interaction on Ti30Ta nanotubes as compared to Ti30 Ta alloy.